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The use of confocal microscopy in experimental studies and clinical practice of an endocrinologist: modern opportunities

Cover Page
  • Authors: Mokrysheva N.G.1, Kiselev S.L.2, Klementieva N.V.3, Gorbacheva A.M.4, Dedov I.I.4,5
  • Affiliations:
    1. Endocrinology research centre
    2. Vavilov Institute of General Genetics of the Russian Academy of Sciences
    3. Privolzhskiy Research Medical University
    4. Endocrinology Research Centre
    5. I.M. Sechenov First Moscow State Medical University
  • Issue: Vol 65, No 3 (2019)
  • Pages: 174-183
  • Section: Reviews
  • URL: https://probl-endojournals.ru/probl/article/view/10140
  • DOI: https://doi.org/10.14341/probl10140
  • Cite item
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Abstract


Confocal microscopy is a modern imaging method that provides ample opportunities for in vitro and in vivo research. The clinical part of the review focuses on well-established techniques, such as corneal confocal microscopy for the diagnosis of diabetic neuropathy or endocrine ophthalmopathy; new methods are briefly described (intraoperative evaluation of tissues obtained by removing pituitary adenomas, thyroid and parathyroid glands). In the part devoted to fundamental research, the use of confocal microscopy to characterize the colocalization of proteins, as well as three-dimensional intracellular structures and signaling pathways in vivo, is considered. Indicators of intracellular calcium are analyzed.


Natalya G. Mokrysheva

Endocrinology research centre

Email: nm70@mail.ru
ORCID iD: 0000-0002-9717-9742
SPIN-code: 5624-3875

Russian Federation, 11, Dm. Ulyanova street, Moscow, 117036

MD, PhD, Professor

Sergey L. Kiselev

Vavilov Institute of General Genetics of the Russian Academy of Sciences

Email: sl_kiselev@yahoo.com
ORCID iD: 0000-0001-7921-6987
SPIN-code: 9311-3403

Russian Federation, 3, Gubkin street, Moscow, 119991

PhD, Professor

Natalia V. Klementieva

Privolzhskiy Research Medical University

Email: nvklementieva@gmail.com
ORCID iD: 0000-0002-2742-372X
SPIN-code: 5318-3774

Russian Federation, 10/1, Minin and Pozharsky square , Nizhny Novgorod, 603005

PhD

Anna M. Gorbacheva

Endocrinology Research Centre

Author for correspondence.
Email: ann.gorbachewa@yandex.ru
ORCID iD: 0000-0003-2669-9457
SPIN-code: 9815-7509

Russian Federation, 11, Dm. Ulyanova street, Moscow, 117036

MD

Ivan I. Dedov

Endocrinology Research Centre; I.M. Sechenov First Moscow State Medical University

Email: dedov@endocrincentr.ru
ORCID iD: 0000-0002-8175-7886
SPIN-code: 5873-2280

Russian Federation, 11, Dm. Ulyanova street, Moscow, 117036; 8-2, Trubetskaya street, Moscow, 119992

MD, PhD, Professor

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Supplementary files

Supplementary Files Action
1. Fig. 1. The results of the study L. Chen et al. [eight]. An example of a change in the concentration of intracellular calcium over time when 80 mM KCl solution in dorsal ganglia neurons is added to the medium. Coloring Fluo-4AM. View (578KB) Indexing metadata
2. Fig. 2. The results of a study by Y. Chen et al. [eight]. An example of a change in the concentration of intracellular calcium when 80 mM KCl solution in dorsal ganglia neurons is added to the medium. Coloring Oregon Green – 488. Сon - control image, High KCl - image under conditions of 80 mM KCl. View (23KB) Indexing metadata
3. Fig. 3. The results of the study L. Mercurio et al. [16]. Fluorescence confocal microscopy: stained with CXCR4 (green label), PLC (red label), cell nuclei (DAPI - blue label). View (48KB) Indexing metadata
4. Fig. 4. Research results J. Walczak et al. [eighteen]. Intracellular distribution of mitochondria (red fluorescence) and expression of mitochondrial stress protein Drp 1 (green fluorescence) in NARP cells. View (29KB) Indexing metadata

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Copyright (c) 2019 Mokrysheva N.G., Kiselev S.L., Klementieva N.V., Gorbacheva A.M., Dedov I.I.

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