Association of the CCR5 gene with type 1 diabetes mellitus

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Abstract


Aim of the study. To elucidate the association between the polymorphous marker A/del132 of the CCR5 gene with type 1 diabetes mellitus. Materials and methods. The study included 177 patients with type 1 diabetes mellitus (DM1) and 408 healthy subjects (ethnic Russians). CR5 alleles and genotypes were identified with the use of the real-time amplification technique. Results. It was shown that the CCR5 allele without 32 base pair deletion (allele A) predominated in both diabetic patients and diabetes-free subjects. The difference between their occurrence in the two groups was insignificant. At the same time, we documented a significant rise in the frequency of del132/del132 genotype in the diabetic patients compared with the healthy subjects (p=0.008). It is concluded that carriers of the CCR5-del32/del32 genotype in the Russian population of Moscow are at high risk of developing type 1 diabetes mellitus.

O I Kopylova

T L Kuraeva

E Iu Lavrikova

E V Titovich

A G Nikitin

G E Smirnova

V A Peterkova

I I Dedov

V V Nosikov

Email: nosikov@genetika.ru

  1. Devendra D., Liu E., Eisenbarth G.S. Type 1 diabetes: recent developments. BMJ 2004; 328: 7442: 750-754.
  2. Baker P.R., Steck A.K. The past, present, and future of genetic associations in type 1 diabetes. Curr Diabet Rep 2011; 11: 445-453.
  3. Haller M.J., Gottlieb P.A., Schatz D.A. Type 1 diabetes intervention trials 2007: where are we and where are we going? Curr Opin Endocrinol Diabet Obes 2007; 14: 4: 283-287.
  4. Chistiakov D.A., Savost'anov K.V., Nosikov V.V. CTLA4 gene polymorphisms are associated with, and linked to, insulin-dependent diabetes mellitus in a Russian population. BMC Genet 2001; 2: 6.
  5. Никитин А.Г., Лаврикова Е.Ю., Серегин Ю.А., Зильберман Л.И., Цитлидзе Н.М., Кураева Т.Л., Петеркова В.А., Дедов И.И., Носиков В.В. Ассоциация полиморфного маркера - 23HphI гена INS с сахарным диабетом 1-го типа. Сахарный диабет 2010; 2: 17-20.
  6. Лаврикова Е.Ю., Никитин А.Г., Серегин Ю.А., Зильберман Л.И., Цитлидзе Н.М., Кураева Т.Л., Петеркова В.А., Дедов И.И., Носиков В.В. Ассоциация полиморфного маркера C1858T гена PTPN22 с сахарным диабетом 1-го типа. Мол биол 2009; 43: 6: 1040-1043.
  7. Никитин А.Г., Лаврикова Е.Ю., Серегин Ю.А., Зильберман Л.И., Цитлидзе Н.М., Кураева Т.Л., Петеркова В.А., Дедов И.И., Носиков В.В. Ассоциация полиморфных маркеров генов ERBB3 и SH2B3 с сахарным диабетом 1-го типа. Мол биол 2010; 44: 2: 257-262.
  8. Allen S.J., Crown S.E., Handel T.M. Chemokine: receptor structure, interactions, and antagonism. Annu Rev Immunol 2007; 25: 787-820.
  9. Rottman J.B., Ganley K.P., Williams K., Wu L., Mackay C.R., Ringler D.J. Cellular localization of the chemokine receptor CCR5: correlation to cellular targets of HIV-1 infection. Am J Path 1997; 151: 1341-1351.
  10. Samson M., Libert F., Doranz B.J., Rucker J., Liesnard C., Farber C-M., Saragosti S., Lapoumeroulie C., Cogniaux J., Forceille C., Muyldermans G., Verhofstede C., Burtonboy G., Georges M., Imai T., Rana S., Yi Y., Smyth R.J., Collman R.G., Doms R.W., Vassart G., Parmentier M. Resistance to HIV-1 infection in Caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene. Nature 1996; 382: 722-725.
  11. Kantarci O.H., Morales Y., Ziemer P.A., Hebrink D.D., Mahad D.J., Atkinson E.J., Achenbach S.J., De Andrade M., Mack M., Ransohoff R.M., Lassmann H., Bruck W., Weinshenker B.G., Lucchinetti C.F. CCR5Δ32 polymorphism effects on CCR5 expression, patterns of immunopathology and disease course in multiple sclerosis. J Neuroimmunol 2005; 169: 1-2: 137-143.
  12. Szalai C., Császár A., Czinner A., Szabó T., Pánczél P., Madácsy L., Falus A. Chemokine receptor CCR2 and CCR5 polymorphisms in children with insulin-dependent diabetes mellitus. Pediatr Res 1999; 46: 1: 82-84.
  13. Lohmann T., Laue S., Nietzschmann U., Kapellen T.M., Lehmann I., Schroeder S., Paschke R., Kiess W. Reduced expression of Th1-associated chemokine receptors on peripheral blood lymphocytes at diagnosis of type 1 diabetes. Diabetes 2002; 51: 8: 2474-2480.
  14. Weber C. Novel mechanistic concepts for the control of leukocyte transmigration: Specialization of integrins, chemokines, and junctional molecules. J Mol Med 2003; 81: 4-19.
  15. Viola A., Molon B., Contento R.L. Chemokines: coded messages for T-cell missions. Front Biosci 2008; 13: 6341-6353.
  16. Muxel S.M., Borelli S.D., Amarante M.K., Voltarelli J.C., Aoki M.N., de Oliveira C.E., Ehara Watanabe M.A. Association study of CCR5 delta 32 polymorphism among the HLA-DRB1 Caucasian population in Northern Paraná, Brazil. J Clin Lab Analyt 2012; 22: 4: 229-233.
  17. Favorova O.O., Favorov A.V., Boiko A.N., Andreewski T.V., Sudomoina M.A., Alekseenkov A.D., Kulakova O.G., Gusev E.I., Parmigiani G., Ochs M.F. Three allele combinations associated with Multiple Sclerosis. BMC Med Genet 2006; 7: 63.
  18. Smyth D.J., Plagnol V., Walker N.M., Cooper J.D., Downes K., Yang J.H., Howson J.M., Stevens H., McManus R., Wijmenga C., Heap G.A., Dubois P.C., Clayton D.G., Hunt K.A., van Heel D.A., Todd J.A. Shared and distinct genetic variants in type 1 diabetes and cCeliac disease. N Engl J Med 2008; 359: 26: 2767-2777.
  19. Gambelunghe G., Ghaderi M., Brozzetti A., Del Sindaco P., Gharizadeh B., Nyren P., Hjelmström P., Nikitina-Zake L., Sanjeevi C.B., Falorni A. Lack of association of CCR2-64I and CCR5-Delta 32 with type 1 diabetes and latent autoimmune diabetes in adults. Hum Immunol 2003; 64: 6: 629-632.
  20. Yang B., Houlberg K., Millward A., Demaine A. Polymorphisms of chemokine and chemokine receptor genes in Type 1 diabetes mellitus and its complications. Cytokine 2004; 26: 3: 114-121.
  21. Johns M.B., Paulus-Thomas J.E. Purification of human genomic DNA from whole blood using sodium perchlorate in place of phenol. Anal Biochem 1989; 180: 2: 276-278.
  22. Никитин А. Калькулятор для расчета статистики в исследованиях "случай-контроль". Интернет 2009; доступен по адресу: http://test.tapotili.ru/calculator_or.php
  23. Koeleman B.P., Lie B.A., Undlien D.E., Dudbridge F., Thorsby E., de Vries R.R., Cucca F., Roep B.O., Giphart M.J., Todd J.A. Genotype effects and epistasis in type 1 diabetes and HLA-DQ trans dimer associations with disease. Genes Immun 2004; 5: 5: 381-388.

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Copyright (c) 2013 Kopylova O.I., Kuraeva T.L., Lavrikova E.I., Titovich E.V., Nikitin A.G., Smirnova G.E., Peterkova V.A., Dedov I.I., Nosikov V.V.

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