Expression of the adiponectin gene (ADIPOQ) in the subcutaneous and visceral fatty tissues and the serum adiponectin level in children

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The primary objective of the present work was to study specific features of adiponectin gene (ADIPOQ) expression in the subcutaneous and visceral fatty tissues along with the serum adiponectin level in children. The secondary objective was to elucidate the relationship between these variables and the basic anthropometric characteristics. The study included a total of 62 patients (31 boys and 31 girls at the age from 2.5 to 18 (median 13.6 (8.5-15.1) years] after they underwent planned surgical interventions. The expression of the adiponectin gene ADIPOQ was determined in paired samples of adipose tissue using the polymerase chain reaction in the real time; in addition, the serum adiponectin levels were measured. The expression of the adiponectin gene ADIPOQ was shown to be unrelated either to the age or to the sex of the children. Nor was there any significant difference between its expression in the subcutaneous and visceral tissues. The highest expression of mRNA encoding for adiponectin was recorded in the children at the Tanner stages 2-3 of sexual development. The ADIPOQ gene expression in the subcutaneous and visceral tissues of overweight children was 22% and 22.6% higher respectively than in the same tissues of normal weight children. Gene expression in the subcutaneous adipose tissue negatively correlated with the serum adiponectin level ( R = –0.38, p = 0.002), BMI SD (R = –0.35, p = 0.004 ), and the waist circumference (R = –0.36, p = 0.004). The results of the study suggest the necessity of further studies to clarify the pathophysiological role of adiponectin in the development of obesity and the related metabolic disturbances.

A V Kosygina


V V Sosunov

V A Peterkova

I I Dedov

  1. Hu E., Liang P., Spiegelman B.M. AdipoQ is a novel adipose-specific gene dysregulated in obesity. J Biol Chem 1996;271:10697-10703.
  2. Maeda K., Okubo K., Shimomura I. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1). Biochem Biophys Res Commun 1996; 221: 286-289.
  3. Nakano Y., Tomita M. et al. Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma. J Biochem (Tokyo) 1996;120:803-812.
  4. Scherer P.E., Williams S. et al. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem 1995;270:26746-26749.
  5. Fain J.N., Bahouth S.W. et al. Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans. Endocrinology 2004; 145: 2273-2282.
  6. Xiaonan L., Lindquist S., Angsten G. et al. Adiponectin and peroxisome proliferator-activated receptor gamma expression in subcutaneous and omental adipose tissue in children. Acta Paediat 2008;97:630-635.
  7. Chandran M., Henry R.R. et al. Adiponectin: more than just another fat cell hormone? Diabet Care 2003;26:2442-2450.
  8. Kadowaki T., Yamauchi T. Adiponectin and Adiponectin Receptors. Endocrine Rev 2005;26:3:439-451.
  9. Adamczak M., Wiecek A., Funahashi T. et al. Decreased plasma adiponectin concentration in patients with essential hypertension. Am J Hypertens 2003;16:72-75.
  10. Arita Y., Kihara S., Ouchi N. Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun 1999;257:79-83.
  11. Choi K.M., Lee J., Lee K.W. et al. Serum adiponectin concentrations predict the developments of type 2 diabetes and the metabolic syndrome in elderly koreans. Clin Endocrinol (Oxf) 2004;61:75-80.
  12. Dzielinska Z., Januszewicz A., Wiecek A. et al. Decreased plasma concentration of a novel anti-inflammatory protein adiponectin in hypertensive men with coronary artery disease. Thromb Res 2003;110:365-369.
  13. Lindsay R.S., Funahashi T., Hanson R.L. et al. Adiponectin and development of type 2 diabetes in the pima indian population. Lancet 2002;360:57-58.
  14. Matsubara M., Maruoka S., Katayose S. Inverse relationship between plasma adiponectin and leptin concentrations in normal-weight and obese women. Eur J Endocrinol 2002;147:173-180.
  15. Matsubara M., Maruoka S., Katayose S. Decreased plasma adiponectin concentrations in women with dyslipidemia. J Clin Endocrinol Metab 2002;87:2764-2769.
  16. Li X., Lindquist S., Chen R. et al. Depot-specific messenger RNA expression of 11 betahydroxysteroid dehydrogenase type 1 and leptin in adipose tissue of children and adults. Int J Obes (London) 2007;31:820-828.
  17. Behre C.J., Gummesson A., Jernås M., Lystig T.C. Dissociation between adipose tissue expression and serum levels of adiponectin during and after diet-induced weight loss in obese subjects with and without the metabolic syndrome. Metabolism 2007;56:8:1022-1028.
  18. Savu M.K., Phillips S.A., Oh D.K. et al. Response of adiponectin and its receptors to changes in metabolic state after gastric bypass surgery: dissociation between adipose tissue expression and circulating levels. Surg Obes Relat Dis 2009;5:2:172-180.
  19. Bottner A., Kratzsch J., Muller G. Gender differences of adiponectin levels develop during the progression of puberty and are related to serum androgen levels. J Clin Endocrinol Metab 2004;89:4053-4061.
  20. Nishizawa H., Shimomura I., Kishida K. Androgens Decrease Plasma Adiponectin, an Insulin-Sensitizing Adipocyte-Derived Protein. Diabetes 2002;51:9:2734-2741.


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Copyright (c) 2010 Kosygina A.V., Sosunov V.V., Peterkova V.A., Dedov I.I.

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